Nocardioides agrisoli sp. nov., isolated from farmland soil

A novel Gram-stain-positive bacterium, designated djl-8^T, was isolated from farmland soil in Nanjing, Jiangsu province, PR China. Cells of strain djl-8^T were aerobic, non-motile, non-spore-forming and rod-shaped. The organism grew at 25-37 °C, pH 5.5-8.0 and 0.5-4.0 % NaCl (w/v). The DNA G+C content was 69.3 mol%. The diagnostic diamino acid in the cell-wall peptidoglycan was LL-2, 6-diaminopimelic acid. The major fatty acids (>5 %) were iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, 10-Me C17 : 0 and C17 : 1ω8c. The respiratory quinone was MK-8 (H4) and the major polar lipids were phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol and unknown phospholipids. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain djl-8^T is a member of the genus Nocardioides and shared the highest similarity with Nocardioides ginkgobilobae SYP-A7303^T (97.1 %), followed by Nocardioides soli mbc-2^T (96.9 %), Nocardioide spyridinolyticus OS4^T (96.6 %) and Nocardioides maradonensis RP-B30^T (96.6 %). Strain djl-8^T exhibited low DNA-DNA relatedness with Nocardioides ginkgobilobae SYP-A7303^T (26.9±2.1 %). On the basis of the morphological, physiological, biochemical and chemotaxonomic characteristics presented in this study, strain djl-8^T represents a novel species of the genus Nocardioides, for which the name Nocardioides agrisoli sp. nov. is proposed. The type strain is djl-8^T (=KCTC 39844^T=CCTCC AB 2017058^T).