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Pitfalls in membrane binding sites studies in post-mortem human brain.

R Cash, R Raisman, P Cervera

    Journal of Receptor Research
    |January 1, 1987
    PubMed
    Summary
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    Neurotransmitter receptor binding in post-mortem brain is reliable up to 24 hours post-mortem. Age and neuroleptic drugs affect receptor characteristics, but agonic conditions do not. This ensures validity in neurological and psychiatric disease research.

    Area of Science:

    • Neuroscience
    • Biochemistry
    • Pharmacology

    Background:

    • Biochemical characterization of neurotransmitter receptor sites in post-mortem human brain is crucial for understanding neurological and psychiatric diseases.
    • Study validity depends on controlling pre-mortem and post-mortem conditions.

    Purpose of the Study:

    • To investigate the impact of age, pre-mortem agonic conditions, drug therapy, and post-mortem delay on neurotransmitter receptor binding sites.
    • To compare experimental strategies for receptor characterization.

    Main Methods:

    • Analysis of five binding sites: alpha-1, alpha-2, beta adrenergic receptors, 5HT-2 serotoninergic receptors, and imipramine binding sites.
    • Controlled assessment of factors including age, pre-mortem anoxia/hypovolemia, drug administration (especially neuroleptics), and post-mortem delay (up to 24 hours).

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  • Comparison of experimental strategies like single point values versus saturation curves.

    • Main Results:

    • Age-related changes in receptor number were observed.
    • Pre-mortem anoxia and hypovolemia did not influence receptor characteristics.
    • Neuroleptic drugs administered pre-mortem affected binding to some post-mortem receptors.
    • Post-mortem delay up to 24 hours showed no effect on binding sites.

    Conclusions:

    • Neurotransmitter receptor studies in post-mortem human brain are valid under controlled conditions, particularly regarding post-mortem delay.
    • Age and pre-mortem drug administration are critical factors influencing receptor binding characteristics.
    • Established protocols can ensure reliable biochemical characterization of receptors for disease research.