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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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The SET1 Complex Selects Actively Transcribed Target Genes via Multivalent Interaction with CpG Island Chromatin
David A Brown1, Vincenzo Di Cerbo1, Angelika Feldmann1
1Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK.
Cell Reports
|September 7, 2017
Summary
The SET1 complex targets gene promoters via CFP1, which reads DNA and H3K4me3. This interaction is crucial for proper gene expression and the vertebrate epigenome.
Area of Science:
- Epigenetics and Gene Regulation
- Molecular Biology
- Chromatin Biology
Background:
- Gene expression regulation relies on chromatin modifications and the promoter epigenome.
- Mechanisms of targeting chromatin-modifying complexes to vertebrate gene promoters are poorly understood.
Purpose of the Study:
- To elucidate how the SET1 complex is targeted to gene promoters in vertebrates.
- To understand the role of CFP1 in SET1 complex targeting and function.
- To investigate the impact of SET1 complex mis-targeting on gene expression.
Main Methods:
- Live-cell imaging
- Functional genomics
- Chromatin immunoprecipitation assays (implied)
Main Results:
- CFP1 targets the SET1 complex to actively transcribed gene promoters.
- CFP1 utilizes multivalent chromatin reading, recognizing non-methylated DNA and H3K4me3.
- CFP1 is essential for SET1 complex occupancy and H3K4me3 deposition.
- Absence of CFP1 leads to perturbed gene expression.
Conclusions:
- CFP1 is a key determinant of SET1 complex targeting to vertebrate gene promoters.
- Multivalent interactions mediated by CFP1 are critical for SET1 complex function.
- Proper SET1 complex targeting and function by CFP1 are essential for a functional vertebrate epigenome.
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