Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

10.2K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
10.2K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.5K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.5K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

7.4K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.4K
Notch Signaling Pathway03:14

Notch Signaling Pathway

6.7K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.7K
Cell Signaling Feedback Loops01:07

Cell Signaling Feedback Loops

7.4K
Positive and negative feedback loops are crucial for regulating biological signaling systems. These feedback loops are processes that connect output signals to their inputs.
Negative feedback loops
Most signaling systems have negative feedback loops that can perform different functions such as output limiter, and adaptation.
Output limiter
Upon receiving an input signal, the cellular response rapidly increases until a threshold is reached. Beyond this threshold, a negative feedback loop...
7.4K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

10.1K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel model of cardiovascular-kidney-metabolic syndrome combining unilateral nephrectomy and high-salt-sugar-fat diet in mice.

Lab animal·2024
Same author

Antibacterial mouthwash alters gut microbiome, reducing nutrient absorption and fat accumulation in Western diet-fed mice.

Scientific reports·2024
Same author

NO-ferroheme is a signaling entity in the vasculature.

Nature chemical biology·2023
Same author

Red blood cells from endothelial nitric oxide synthase-deficient mice induce vascular dysfunction involving oxidative stress and endothelial arginase I.

Redox biology·2023
Same author

Hemoglobin β93 Cysteine Is Not Required for Export of Nitric Oxide Bioactivity From the Red Blood Cell.

Circulation·2019
Same author

AMP-activated protein kinase activation and NADPH oxidase inhibition by inorganic nitrate and nitrite prevent liver steatosis.

Proceedings of the National Academy of Sciences of the United States of America·2018

Related Experiment Video

Updated: Feb 23, 2026

Measurement of Heme Synthesis Levels in Mammalian Cells
09:43

Measurement of Heme Synthesis Levels in Mammalian Cells

Published on: July 9, 2015

12.6K

The NO-heme signaling hypothesis.

Andrei L Kleschyov1

  • 1Laboratory of Biophysics, Freiberg Instruments GmbH, 09599 Freiberg, Germany.

Free Radical Biology & Medicine
|September 7, 2017
PubMed
Summary

This study proposes that cells generate mobile nitric oxide-heme (NO-heme) species. These NO-heme complexes facilitate safer, efficient cell signaling, impacting various biological processes and offering new therapeutic avenues.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Physiology

Background:

  • Nitric oxide (NO) synthase (NOS) plays a crucial role in cellular signaling.
  • The canonical NOS/NO pathway involves NO activating soluble guanylyl cyclase (sGC) to produce cyclic GMP.
  • Fundamental aspects of NOS/NO signaling remain incompletely understood.

Purpose of the Study:

  • To propose a novel mechanism of NO signaling involving mobile NO-heme species.
  • To explore the implications of NO-heme complexes in cellular communication and regulation.
  • To identify potential new applications in diagnostics and therapeutics.

Main Methods:

  • Theoretical analysis and review of existing literature.
  • Hypothesizing the formation and function of mobile NO-heme species.
Keywords:
HemeNO-heme signalingNitric oxideS-nitrosationSoluble guanylyl cyclase

More Related Videos

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

18.8K
Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
04:01

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

2.0K

Related Experiment Videos

Last Updated: Feb 23, 2026

Measurement of Heme Synthesis Levels in Mammalian Cells
09:43

Measurement of Heme Synthesis Levels in Mammalian Cells

Published on: July 9, 2015

12.6K
Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

18.8K
Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
04:01

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

2.0K
  • Connecting NO-heme signaling to known biological phenomena.

    • Main Results:

    • Cells may dynamically generate mobile/exchangeable NO-ferroheme species.
    • These NO-heme complexes can activate sGC and regulate other biomolecules.
    • NO-heme signaling offers a mechanism for safe, efficient, and coordinated signal delivery.

    Conclusions:

    • NO-heme signaling provides a new perspective on NOS/NO pathways.
    • This mechanism may explain various NO-related phenomena, including vasodilation and protein nitrosation.
    • The findings predict new discoveries and therapeutic strategies for NO-related conditions.