Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.1K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.1K
CRISPR01:59

CRISPR

58.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.2K
Homologous Recombination02:31

Homologous Recombination

64.2K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
64.2K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.9K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Point-of-care-ultrasound in in-hospital cardiac arrest.

Resuscitation plus·2026
Same author

Sepsis and acute kidney injury-related mortality in the U.S.: National trends and disparities (1999-2023).

Medicine·2026
Same author

Antifungal potential of biocontrol agents against Penicillium georgiense causing onion mold in Pakistan.

International microbiology : the official journal of the Spanish Society for Microbiology·2026
Same author

Red Blood Cell Transfusion in Neurocritical Patients: An Updated Systematic Review and Meta-Analysis of Mortality and Major Complications.

Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion·2026
Same author

Aitomia: An Agentic Framework for AI-Driven Atomistic and Quantum Chemical Simulations.

Journal of chemical theory and computation·2026
Same author

Dual-Layer PDMS/Polysulfone Composite Membranes Incorporating Cu-MOF-74 for Enhanced CO<sub>2</sub> Capture Performance.

Polymers·2026
Same journal

Correction: Wang et al. Phosphatidylserine Decarboxylase Promotes Ferroptosis Through STAT3/GPX4 Signaling in Gastric Cancer. <i>Curr. Issues Mol. Biol.</i> 2026, <i>48</i>, 300.

Current issues in molecular biology·2026
Same journal

Exploring the Relationship Between Protein-Level Ratios (rQLTs) and Duodenal Ulcer.

Current issues in molecular biology·2026
Same journal

Metformin as an Innate Immune Modulator: Metabolic and Epigenetic Reprogramming of Innate Immune Cells and Therapeutic Implications.

Current issues in molecular biology·2026
Same journal

Comprehensive Bioinformatic Characterization of CD70, CD80, and TIGIT as Diagnostic, Prognostic, and Immune Biomarkers in Pan-Cancer.

Current issues in molecular biology·2026
Same journal

Genome-Wide Identification and Expression Analysis of the Thaumatin-like Protein Genes in <i>Filipendula ulmaria</i> under <i>Bipolaris sorokiniana</i> Infection.

Current issues in molecular biology·2026
Same journal

Recent Dominant Transposition Events Affect Gene Regulatory Regions, but Not Coding Sequences, in Polar and Brown Bear Genomes.

Current issues in molecular biology·2026
See all related articles

Related Experiment Video

Updated: Feb 23, 2026

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
11:37

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

Published on: February 26, 2019

10.4K

Improving CRISPR-Cas9 On-Target Specificity.

Muhammad Jamal1, Arif Ullah2, Muhammad Ahsan1

  • 1State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, The People's Republic of China, and College of Animal Science and Medicine, Huazhong Agricultural University, Wuhan, The People's Republic of China.

Current Issues in Molecular Biology
|September 8, 2017
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas9 gene editing offers precise genome targeting but requires high on-target specificity for therapeutic use. Recent advancements in engineered nucleases, gRNA design, and off-target detection significantly improve CRISPR specificity for clinical applications.

More Related Videos

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

1.6K
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

13.3K

Related Experiment Videos

Last Updated: Feb 23, 2026

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
11:37

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

Published on: February 26, 2019

10.4K
CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

1.6K
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

13.3K

Area of Science:

  • Molecular Biology
  • Medical Genetics
  • Biotechnology

Background:

  • CRISPR-Cas9 technology enables facile genome targeting in various organisms.
  • On-target specificity is critical for therapeutic applications of CRISPR-Cas9.
  • Off-target cleavage by Cas9 nucleases remains a concern for clinical translation.

Purpose of the Study:

  • To summarize recent advances in CRISPR-Cas9 technology.
  • To highlight improvements in specificity for genome editing tools.
  • To discuss the potential of enhanced CRISPR tools in clinical research.

Main Methods:

  • Development of engineered nucleases with improved specificity.
  • Optimization of guide RNA (gRNA) selection strategies.
  • Exploration of novel Cas9 orthologs for enhanced targeting.
  • Advancement of methods for detecting and screening off-target effects.

Main Results:

  • Current research demonstrates significantly improved specificity of CRISPR technology.
  • Engineered nucleases, optimized gRNAs, and novel Cas9 orthologs enhance on-target activity.
  • Advanced screening methods aid in identifying and mitigating off-target effects.

Conclusions:

  • CRISPR-Cas9 technology has been revolutionized by advancements enhancing its specificity.
  • These improvements are equipping genome editing tools for clinical research applications.
  • Further optimization focusing on specificity is crucial for perfecting CRISPR-based therapies.