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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Homologous Recombination02:31

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Updated: Feb 23, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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CRISPR Mediated Genome Engineering and its Application in Industry.

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The CRISPR-Cas9 genome editing tool precisely modifies DNA using guide RNA. This technology revolutionizes biomedical science, offering applications in disease treatment, drug design, and agriculture.

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Area of Science:

  • Biotechnology
  • Genomics
  • Molecular Biology

Background:

  • CRISPR-Cas9 is a revolutionary genome engineering technology.
  • It enables precise DNA modification using a guide RNA (gRNA) and Cas9 nuclease.
  • This tool has broad applications across various scientific disciplines.

Purpose of the Study:

  • To introduce the Cas9-mediated genome-editing tool.
  • To summarize recent advances in CRISPR/Cas9 technology.
  • To discuss applications in disease treatment, drug design, agriculture, and more.

Main Methods:

  • Review of CRISPR/Cas9 technology and its advancements.
  • Discussion of applications in engineering genomes of diverse organisms.
  • Exploration of CRISPR/Cas9 utility in various scientific fields.

Main Results:

  • CRISPR/Cas9 is a rapid, efficient, and versatile genome engineering tool.
  • It has diverse applications including treatment of fungal and viral diseases.
  • The technology offers advantages in drug design, animal model creation, and food/agricultural sciences.

Conclusions:

  • CRISPR/Cas9 technology is transforming biomedical and biotechnological research.
  • It facilitates the development of strains with desired traits for industrial and medical uses.
  • This technology is crucial for investigating genome function and driving innovation.