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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Automated cell counts on CSF samples: A multicenter performance evaluation of the GloCyte system.

E A Hod^1,2, C Brugnara³, M Pilichowska⁴

¹Department of Pathology and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY, USA.

International Journal of Laboratory Hematology

|September 8, 2017

Summary

The GloCyte System accurately counts low cell numbers in cerebrospinal fluid (CSF), offering a precise automated alternative to manual cell counting. This system is suitable for all CSF samples, including those with normal cell counts.

Keywords:

automated cell counters cerebrospinal fluid testing laboratory testing red cell counts white cell counts

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Area of Science:

Clinical Chemistry
Hematology
Medical Diagnostics

Background:

Manual cell counting in cerebrospinal fluid (CSF) is labor-intensive due to the unreliability of automated methods at low cell counts.
Laboratories often maintain manual CSF counts despite automated methods for other body fluids.

Purpose of the Study:

To evaluate the GloCyte System, an automated cell counter, for accuracy and precision in enumerating low cell counts in CSF.
To determine if the GloCyte System can replace manual CSF cell counts.

Main Methods:

A multicenter clinical trial using residual CSF samples from adult and pediatric patients.
Comparison of cell counts obtained via the manual hemocytometer method and the GloCyte System.
Determination of the GloCyte System's limits of detection, quantitation, precision, and accuracy.

Main Results:

The GloCyte System detected as low as 1 cell/μL and reliably counted 3 total nucleated cells/μL and 2 red blood cells/μL.
High correlation (>97%) was observed between GloCyte and hemocytometer counts, even at very low cell concentrations.
The total coefficient of variation for the GloCyte System was under 20%.

Conclusions:

The GloCyte instrument provides precise, accurate, and stable cell counts for CSF samples.
Automated enumeration of very low cell numbers in CSF is feasible with the GloCyte System.
The GloCyte System is a viable alternative to manual methods for all CSF cell counting needs.