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Viability of Allogeneic Bone Marrow Stromal Cells following Local Delivery into Patella Tendon in Rabbit Model.
Hong Wei Ouyang1, James C H Goh1,2, Eng Hin Lee1
1Department of Orthopaedic Surgery, National University of Singapore, Singapore 119260.
Cell Transplantation
|September 8, 2017
Summary
Bone marrow stromal cells (bMSCs) show potential for tendon repair. Labeled bMSCs remained viable in rabbit patellar tendon defects for up to 8 weeks, indicating their suitability for regenerative therapies.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Orthopedic Surgery
Background:
- Bone marrow stromal cells (bMSCs) are investigated for tendon repair and regeneration.
- Ensuring the survival of implanted cells is crucial for successful tendon healing.
Purpose of the Study:
- To determine the in vivo fate and viability of allogeneic bone marrow stromal cells (bMSCs) after implantation into rabbit patellar tendon defects.
- To assess the survival duration and morphological changes of bMSCs over time.
Main Methods:
- Rabbit patellar tendon window defects were created and treated with GFP- or CFDA-labeled bMSCs embedded in fibrin glue.
- Cell viability was assessed using confocal microscopy at 2, 3, 5, and 8 weeks post-implantation.
- Morphological changes of bMSCs were documented.
Main Results:
- Labeled bMSCs demonstrated sustained viability within the tendon defect site for at least 8 weeks.
- Cell morphology transitioned from round at 2 weeks to spindle-shaped by 5 weeks post-implantation.
- The study confirmed the long-term survival of bMSCs in a tendon defect model.
Conclusions:
- Allogeneic bone marrow stromal cells remain viable for extended periods after implantation into tendon defects.
- These findings support the potential of bMSCs to contribute to neotendon formation and remodeling.
- Sustained bMSC viability suggests their therapeutic promise for tendon repair and regeneration.
