Related Experiment Video

Updated: Feb 23, 2026

Isolation and Culture Expansion of Tumor-specific Endothelial Cells
10:15

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Published on: October 14, 2015

11.7K

C/EBP-δ positively regulates MDSC expansion and endothelial VEGFR2 expression in tumor development

Yongfen Min1, Jingdong Li2,3, Peng Qu1

  • 1Center for Cancer Research, National Cancer Institutes, Frederick, MD 21702, USA.

Oncotarget
|September 9, 2017
PubMed

Insights

The study identifies C/EBP-δ as a key regulator of myeloid-derived suppressor cells (MDSCs) and vascular endothelial growth factor receptor 2 (VEGFR2) in the tumor microenvironment. Targeting C/EBP-δ may offer a novel cancer therapy approach.

Area of Science:

  • Immunology
  • Cancer Biology
  • Molecular Biology

Background:

  • The tumor microenvironment involves vascular endothelial cells and myeloid-derived suppressor cells (MDSCs).
  • Targeting these components is a strategy for cancer therapy.
  • A common mediator regulating both is yet to be fully elucidated.

Purpose of the Study:

  • To identify a common mediator regulating both vascular endothelial cells and MDSCs in tumor development.
  • To investigate the role of C/EBP-δ in tumor progression and its mechanisms of action.

Main Methods:

  • Genetic deletion of C/EBP-δ in mice.
  • Analysis of MDSC expansion and Gr-1+CD11b+ cell production.
  • Assessment of tumor angiogenesis and growth.
  • Investigation of C/EBP-δ expression and function in vascular endothelial cells, including VEGFR2 regulation.
  • Chromatin immunoprecipitation to assess C/EBP-δ binding to the VEGFR2 promoter.

Main Results:

  • C/EBP-δ is elevated in tumor-derived MDSCs and promotes their expansion under tumor conditions.
  • Loss of C/EBP-δ reduces tumor angiogenesis and growth.
  • C/EBP-δ regulates vascular endothelial cell motility, network formation, and sprouting.
  • C/EBP-δ directly regulates VEGFR2 expression in endothelial cells via promoter recruitment.

Conclusions:

  • C/EBP-δ is a crucial mediator promoting tumor development by regulating both MDSC expansion and VEGFR2 expression in endothelial cells.
  • Targeting C/EBP-δ presents a potential dual-action strategy for cancer therapy.
Keywords:
C/EBP-δMDSCsVEGFR2angiogenesiscancer

More Related Videos

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells08:54

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

Published on: July 20, 2014

14.5K
A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors09:04

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

Published on: March 15, 2016

10.2K

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.8K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.7K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
8.2K
The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
8.0K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.9K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K