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Reusable Silicon-Based Surface-Enhanced Raman Scattering Ratiometric Aptasensor with High Sensitivity, Specificity,
Huayi Shi1, Na Chen1, Yuanyuan Su1
1Laboratory of Nanoscale Biochemical Analysis, Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University , Suzhou, Jiangsu 215123, China.
This study introduces a novel ratiometric silicon surface-enhanced Raman scattering (SERS) aptasensor for highly sensitive and accurate detection of adenosine triphosphate (ATP). The developed aptasensor demonstrates excellent reproducibility and recyclability for reliable ATP quantification.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Analytical Chemistry
Background:
- Adenosine triphosphate (ATP) is crucial for cellular processes and signaling.
- Accurate ATP detection is vital for biological research.
- Existing surface-enhanced Raman scattering (SERS) aptasensors face challenges in reliable ATP quantification.
Purpose of the Study:
- To develop a ratiometric silicon SERS aptasensor for sensitive and accurate ATP detection.
- To overcome limitations in current ATP quantification methods using aptasensors.
- To establish a reliable and reproducible platform for ATP detection.
Main Methods:
- Fabrication of a ratiometric silicon SERS aptasensor using silver nanoparticles (Ag NPs) on a silicon wafer.
- Functionalization with double-stranded DNA (dsDNA) incorporating a Cy3-labeled DNA segment and a ROX-labeled DNA segment.
- Utilizing the dissociation of the ROX-labeled segment upon ATP binding to generate a ratiometric signal (IROX/ICy3).
Main Results:
- The aptasensor achieved ultrahigh sensitivity with a limit of detection (LOD) of 9.12 pM.
- Excellent reproducibility was demonstrated with a relative standard deviation (RSD) of less than 4%.
- The sensor exhibited good recyclability with an RSD of approximately 9.3% over three cycles.
Conclusions:
- The developed ratiometric silicon SERS aptasensor offers a promising platform for accurate and reliable ATP detection.
- This method provides superior sensitivity and reproducibility compared to previously reported ATP SERS aptasensors.
- The aptasensor's performance highlights its potential for various biological and medical applications requiring ATP monitoring.
