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Related Concept Videos

Kendall's Tau Test01:16

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Kendall's tau test, also known as the Kendall rank coefficient test, is a nonparametric method for assessing association between two variables. This test is particularly useful for identifying significant correlations when the distributions of the sample and population are unknown. Developed in 1938 by the British statistician Sir Maurice George Kendall, the tau coefficient (denoted as τ) serves as a rank correlation coefficient, with values ranging from -1 to +1.
A τ value of +1 indicates...
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Related Experiment Video

Updated: Feb 23, 2026

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening
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Methods related to studying tau fragmentation.

Adriana Ferreira1, Sana Afreen1

  • 1Feinberg School of Medicine, Northwestern University, Chicago, IL, United States.

Methods in Cell Biology
|September 9, 2017
PubMed
Summary

Tau fragmentation, a key modification in neurodegenerative diseases like Alzheimer's disease (AD), generates toxic fragments. These fragments contribute to neuronal death and synapse loss, highlighting their pathological role.

Keywords:
CalpainCaspasesCell viability assaysEndopeptidasesNeuronal nucleofectionProtease activity assaysTau cleavage

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pathology

Background:

  • Posttranslational modifications of tau protein are linked to neuronal degeneration.
  • Tau fragmentation is observed in Alzheimer's disease (AD) brains and models.
  • Specific tau fragments resulting from protease activity remain understudied.

Purpose of the Study:

  • To investigate the role of tau fragmentation in neurodegeneration.
  • To characterize toxic tau fragments generated by specific proteases.
  • To develop methods for studying tau fragment effects on neuronal viability.

Main Methods:

  • Protease activity assays were employed.
  • Methods to assess the impact of tau fragments on neuronal viability were described.

Main Results:

  • Enhanced or abnormal activity of calpain, caspase 2, caspase 3, and asparagine endopeptidase leads to toxic tau fragment formation.
  • These tau fragments induce neuronal death, synapse loss, and behavioral deficits.

Conclusions:

  • Tau fragmentation by specific proteases generates neurotoxic species.
  • These findings provide insights into the mechanisms of neurodegeneration in AD and related disorders.
  • The described methods facilitate further research into tauopathies.