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Tissue alkalosis in cold-ischemia time.

V Denninghoff1,2, E H R Olivieri3,4, C Fresno5

  • 1Department of Pathology, A. C. Camargo Cancer Center, São Paulo, Brazil. vcdennin@yahoo.com.ar.

Scientific Reports
|September 9, 2017
PubMed
Summary

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This summary is machine-generated.

Controlling pre-analytical factors is crucial for human biospecimens. This study reveals tissue alkalosis in mouse liver, kidney, and lung samples during cold ischemia, potentially impacting gene expression and highlighting the need for pH regulation.

Area of Science:

  • Biomedical research
  • Pre-analytical factor analysis
  • Tissue physiology

Background:

  • Control of pre-analytical factors in human biospecimens is essential for health research.
  • Limited research exists on cold ischemia's impact on tissue pH, with only two prior studies using post-mortem brain samples.

Purpose of the Study:

  • To investigate and quantify pH variations over time in mouse liver, kidney, and lung samples subjected to cold ischemia.
  • To explore the potential link between tissue alkalosis during cold ischemia and gene expression changes.
  • To emphasize the importance of tissue pH regulation post-organ removal for minimizing biological stress in human samples.

Main Methods:

  • Mice were used as a model system.
  • Tissue samples (liver, kidney, lung) were collected.

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  • pH measurements were taken over varying periods of cold ischemia.
  • Data was analyzed using a third-order polynomial model to describe pH variations.

    • Main Results:

    • Significant pH variations were observed in liver, kidney, and lung tissues over time during cold ischemia.
    • A trend towards tissue alkalosis was noted with increasing cold ischemia time.
    • The observed pH changes followed a third-order polynomial model.

    Conclusions:

    • Cold ischemia induces pH variations in mouse liver, kidney, and lung tissues.
    • Tissue alkalosis during cold ischemia may be a contributing factor to observed gene expression alterations.
    • Implementing tissue pH regulation strategies after organ removal could reduce biological stress in human tissue samples for research.