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Updated: Feb 23, 2026
Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts
Published on: August 13, 2014
Tissue alkalosis in cold-ischemia time
V Denninghoff1,2, E H R Olivieri3,4, C Fresno5
1Department of Pathology, A. C. Camargo Cancer Center, São Paulo, Brazil. vcdennin@yahoo.com.ar.
Abstract:
The control of pre-analytical-factors in human biospecimens collected for health research is currently required. Only two previous reports using post-mortem brain samples have tried to address the impact of cold-ischemia on tissue pH. Here we report pH variations according to time (third-order polynomial model) in mice for liver, kidney and lung samples. Tissue alkalosis in cold-ischemia time may be an underlying mechanism of gene expression changes. Therefore, tissue-pH regulation after organ removal may minimize biological stress in human tissue samples.
