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BRAF mutation in colorectal carcinomas with signet ring cell component.
1Department of Molecular Biology and Genetic, Ahi Evran University, Kirsehir 40100, Turkey.
Cancer Biology & Medicine
|September 9, 2017
Summary
BRAF V600E mutations are common in colorectal cancer (CRC) with signet ring cells. This mutation appears regardless of the percentage of signet ring cells present in the tumor.
Area of Science:
- Oncology
- Molecular Biology
- Gastroenterology
Background:
- Signet ring cell carcinoma is a rare colorectal cancer (CRC) subtype.
- The BRAFV600E mutation is associated with CRC, particularly this subtype.
Purpose of the Study:
- To investigate BRAF V600E mutation frequencies in CRCs with varying signet ring cell components.
- To determine the relationship between BRAF mutation and clinicopathologic parameters in these tumors.
Main Methods:
- Tumors were categorized based on the percentage of signet ring cells (0%-9%, 10%-24%, 25%-49%, >50%).
- Genomic DNA was analyzed for BRAF V600E mutations using polymerase chain reaction-restriction fragment length polymorphism and Sanger sequencing.
Main Results:
- 11 out of 28 CRCs (39.3%) exhibited BRAF V600E mutation.
- BRAF mutations were observed in 36.8% of tumors with <50% signet ring cells and 44.4% with >50% signet ring cells.
- Mutation rates were similar across different percentages of signet ring cell presence (<10% and >10%).
Conclusions:
- BRAF mutation is closely associated with the presence of malignant signet ring cells in colorectal cancer.
- The percentage of signet ring cells does not appear to influence the presence of BRAF mutations.
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