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Updated: Feb 23, 2026

Manipulating the Murine Lacrimal Gland
Published on: November 18, 2014
TGF-β1 attenuated branching morphogenesis of embryonic murine submandibular gland through Smad3 activation
P Gao1,2, X-H Qiao1,2, L-M Gou1,2
1State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Abstract:
Transforming growth factor-β1 (TGF-β1) plays several crucial regulatory roles in multiple physiological and pathological processes. The aim of this work was to investigate the role of TGF-β1 in branching morphogenesis of salivary gland. We harvested and cultured submandibular salivary glands (SMGs) from murine embryos, which were then treated with exogenous TGF-β1, or its neutralized antibody, Smad3 inhibitor, or Smad3 small interfering RNA (siRNA). Our results suggested that TGF-β1 attenuated branching morphogenesis of embryonic murine SMG via Smad3 activation, thus playing a negative regulatory role in salivary gland development.
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