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Updated: Feb 23, 2026

Characterization of pH-Dependent Reversible Self-Assembly of Amyloid Beta 1-40-Coated Gold Colloids
Published on: March 21, 2025
An Anion-π Interaction Strongly Stabilizes the β-Sheet Protein WW
Mason S Smith1, Eliza E K Lawrence1, Wendy M Billings1
1Department of Chemistry and Biochemistry, Brigham Young University , Provo, Utah 84602, United States.
Abstract:
Anions have long been known to engage in stabilizing interactions with electron-deficient arenes. However, the precise nature and energetic contribution of anion-π interactions to protein stability remains a subject of debate. Here, we show that placing a negatively charged Asp in close proximity to electron-rich Phe in a reverse turn within the WW domain results in a favorable interaction that increases WW conformational stability by -1.3 kcal/mol.
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