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Carnosol inhibits Hedgehog signaling pathway in both LNCaP and DU145 prostate cancer cell lines

H Gao1, Q Song2, J Yang2

  • 1Department of Urology, the Dalian Central Hospital, Dalian, China.

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|September 9, 2017
PubMed

Insights

Carnosol inhibits prostate cancer cell growth and induces apoptosis by downregulating the Hedgehog (HH) signaling pathway. This compound shows potential as a therapeutic agent, particularly for hormone-independent prostate cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • The Hedgehog (HH) signaling pathway is implicated in prostate cancer development.
  • Understanding the molecular mechanisms of prostate cancer is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the effect of carnosol on the Hedgehog (HH) signaling pathway in human prostate cancer cells.
  • To evaluate carnosol's potential as a therapeutic agent for both hormone-dependent and hormone-independent prostate cancer.

Main Methods:

  • Immunohistochemistry was used to detect Gli1 and Shh expression in prostate cancer tissues.
  • MTT assay, qRT-PCR, and Western blot were employed to assess cell survival and gene/protein expression.
  • Caspase-3 activity assay was performed to determine apoptosis levels.

Main Results:

  • Sonic hedgehog (Shh) and glioma-associated oncogene homolog 1 (Gli1) were upregulated in prostate cancer tissues.
  • Carnosol inhibited cell survival and proliferation in both LNCaP and DU145 cell lines in a dose-dependent manner.
  • Carnosol downregulated Shh and Gli1 expression and increased caspase-3 activity, indicating apoptosis induction.

Conclusions:

  • Carnosol inhibits prostate cancer cell proliferation and induces apoptosis, potentially through the inhibition of the HH signaling pathway.
  • While carnosol demonstrated stronger effects on hormone-dependent cells, it shows promise as a therapeutic agent for hormone-independent prostate cancer.
Keywords:
CarnosolGli1Hedgehog signaling pathwayProstate cancer​​Shh.

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