Electrochemical nitric oxide biosensor based on amine-modified MoS₂/graphene oxide/myoglobin hybrid

Nitric oxide (NO) is one of the most important molecules in living things due to its role as a signaling molecule in influencing pathological and physiological mechanisms including neurotransmission. In this study, the electrochemical biosensor based on the amine-modified molybdenum disulfide nanoparticles (MoS₂), graphene oxide (GO) and myoglobin (Mb) hybrid material (amine-modified MoS₂/GO/Mb hybrid) is developed to achieve the accurate detection of NO with electrochemical signal improvement. For the first time, the synthesis of MoS₂ accompanying the amine-modification of the surface of MoS₂ is done to hybridize with GO efficiently through the short linkage. After the amine-modification of MoS₂, it is enclosed with GO directly (amine-modified MoS₂/GO). Then, Mb which can induce the reduction of NO is immobilized on the amine-modified MoS₂/GO to fabricate the amine-modified MoS₂/GO/Mb hybrid for NO detection. The prepared hybrid shows the signal improved redox properties relative to the result of the electrode prepared without hybrid. Furthermore, upon addition of NO, the electrode prepared with hybrid shows the improved amperometric response compared with that of the electrode without hybrid. This amine-modified MoS₂/GO/Mb hybrid can be used in the development of the biosensor platform accompanying the electrochemical signal improvement and accurate detection of target materials.