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Differential expression and modulation of Thy-1 on myoblast clones
J S Schweitzer1, S J Kaufman, M A Dichter
1Neuroscience Department, Children's Hospital Medical Center, Boston, Massachusetts 02115.
Experimental Cell Research
|September 1, 1987
Summary
Fibroblasts reduce Thy-1 antigen expression in rat skeletal muscle myoblasts. Two myoblast populations exist: one responsive to the Thy-1-modulating factor (TMF), and another insensitive fibroblast-like type.
Area of Science:
- Cell Biology
- Developmental Biology
- Muscle Biology
Background:
- Fibroblasts modulate Thy-1 antigen expression in rat skeletal muscle myoblasts.
- Previous studies showed reduced Thy-1 antigen expression when myoblasts are co-cultured with fibroblasts or conditioned media.
Purpose of the Study:
- To identify distinct myoblast populations based on their response to a Thy-1-modulating factor (TMF).
- To investigate the stability and characteristics of these myoblast subpopulations.
Main Methods:
- Clonal density culture of rat skeletal muscle myoblasts.
- Exposure to Thy-1-modulating factor (TMF).
- Assessment of Thy-1 antigen expression and cell morphology.
Main Results:
- Two distinct myoblast populations were identified based on TMF responsiveness.
- The majority of myoblasts were TMF-responsive, showing modulated Thy-1 expression.
- A subpopulation of TMF-insensitive myoblasts exhibited fibroblast-like morphology and formed contractile myotubes.
Conclusions:
- Rat skeletal muscle myoblasts comprise distinct subsets with stable, differing responses to TMF.
- These findings suggest stable clonal phenotypes within the myogenic lineage, influenced by fibroblast-derived factors.