Optimized efflux assay for the NorA multidrug efflux pump in Staphylococcus aureus
Related Experiment Video
Updated: Feb 23, 2026
Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds
Published on: September 8, 2021
Saskia Zimmermann1, Lorena Tuchscherr1, Jürgen Rödel2
1Institute of Medical Microbiology, Jena University Hospital, Am Klinikum 1, D-07747 Jena, Germany; Jena University Hospital, Integrated Research and Treatment Center, Center for Sepsis Control and Care (CSCC), Am Klinikum 1, D-07747 Jena, Germany.
Abstract:
Real-time fluorescent efflux assays are commonly used for measuring the efflux of bacterial pumps. Here we describe an optimized protocol for the NorA efflux pump in S. aureus using DiOC3 instead of ethidium bromide. Glucose and sodium formate were tested as energy carriers. This novel method is fast and reproducible.
More Related Videos
10:00Targeting Biofilm Associated Staphylococcus aureus Using Resazurin Based Drug-susceptibility Assay
Published on: May 5, 2016
05:06Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
Published on: January 5, 2024