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Updated: Feb 23, 2026

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Published on: May 10, 2018
Adsorption effects of the doping relevant peptides Insulin Lispro, Synachten, TB-500 and GHRP 5
Péter Judák1, Peter Van Eenoo1, Koen Deventer1
1Ghent University, Department of Clinical Chemistry, Microbiology and Immunology, Doping Control Laboratory, Technologiepark 30 B, B-9052 Zwijnaarde, Belgium.
Abstract:
The tendency of peptides to adsorb to surfaces can raise a concern in variety of analytical fields where the qualitative/quantitative measurement of low concentration analytes (ng/mL-pg/mL) is required. To demonstrate the importance of using the optimal glassware/plasticware, four doping relevant model peptides (GHRP 5, TB-500, Insulin Lispro, Synachten) were chosen and their recovery from various surfaces were evaluated. Our experiments showed that choosing expensive consumables with low-bind characteristics is not beneficial in all cases. A careful selection of the consumables based on the evaluation of the physico/chemical features of the peptide is recommended.
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