Related Experiment Video
Updated: Feb 23, 2026

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
Published on: June 10, 2021
New Colorimetric and Fluorometric Fluoride Ion Probe Based on Anthra[1,9-cd]pyrazol-6(2H)-one
Yang Hu1, Yan-Yan Liu1, Qiao Li1
1Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Hubei Collaborative Innovation Center for Rare Metal Chemistry, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi, 435002, People's Republic of China.
Abstract:
New colorimetric and fluorometric fluoride ion probe, anthra[1,9-cd]pyrazol- 6(2H)-one (1), was synthesized by one-step condensation. The probe 1 shows F--selective color change from colorless to pink and appearance of red fluorescence. The fluorescence quantum yield of free probe 1 in DMSO was calculated to be 0.03. After addition of 15 equiv. of F-, its fluorescence quantum yield can be increased to 0.37. The analytical detection limit for F- was 2.8 × 10- 7 M. 1H NMR analysis and DFT calculation show that the F--induced colorimetric and fluorometric responses of 1 are driven by deprotonation process. Graphical Abstract.
Related Concept Videos
Fluorescence and Phosphorescence: Instrumentation
Photoluminescence: Applications
Atomic Fluorescence Spectroscopy
Immunofluorescence Microscopy
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

