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Efficient differentiation of Corynebacterium striatum, Corynebacterium amycolatum and Corynebacterium xerosis
Carolina S Santos1, Juliana N Ramos2, Veronica V Vieira3
1Institute of Health Sciences, Federal University of Bahia (UFBA), Salvador, BA, Brazil.
Abstract:
A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226bp, 434bp and 106bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS.
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