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Isolation, Transfection, and Culture of Primary Human Monocytes
Published on: December 16, 2019
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SERPING1 mRNA overexpression in monocytes from HIV+ patients
C Sanfilippo1, D Cambria1, A Longo1
1Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
Summary
SERPING1 is upregulated in monocytes of HIV-1 patients, correlating with viral load. This suggests SERPING1 and its network are key in the innate immune response to restrict HIV-1 infection.
Area of Science:
- Immunology
- Virology
Background:
- The complement system is crucial for immunity and is activated by HIV-1.
- SERPING1, a protease inhibitor, targets the C1 complex in the classical complement pathway.
Purpose of the Study:
- To investigate the role of SERPING1 in HIV-1 infection.
- To analyze SERPING1 mRNA modulation in monocytes of HIV-1-infected individuals.
Main Methods:
- Analysis of GEO dataset microarrays.
- Validation using monocytes from HIV-1+ patients and healthy controls treated with interferons (IFNs).
Main Results:
- SERPING1 mRNA is overexpressed in monocytes from HIV-1+ patients, correlating with viral load and inversely with CD4+ T-cell count.
- Anti-retroviral therapy reduced SERPING1 mRNA levels ex vivo.
- IFN treatment upregulated SERPING1 mRNA expression, and IFITM1 expression correlated positively with SERPING1.
Conclusions:
- SERPING1 and its network are integral to the innate immune system's restriction of HIV-1.
- SERPING1 may serve as a biomarker for HIV-1 infection and disease progression.
