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Related Concept Videos

Amperometry: Overview01:10

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Surface-modified CMOS IC electrochemical sensor array targeting single chromaffin cells for highly parallel

Meng Huang1, Joannalyn B Delacruz1, John C Ruelas2

  • 1School of Applied and Engineering Physics, Cornell University, Ithaca, NY, 14853, USA.

Pflugers Archiv : European Journal of Physiology
|September 11, 2017
PubMed
Summary

This study presents a low-cost method for single-cell amperometry using microwells on CMOS sensor arrays. This technique enhances cell targeting efficiency for reliable recordings of quantal release events from chromaffin cells.

Keywords:
AmperometryBiosensorCell trappingHigh throughputOn-chip recordingPost-fabricationShift electrode

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Area of Science:

  • Neuroscience
  • Electrochemistry
  • Biotechnology

Background:

  • Amperometry is crucial for studying quantal release from chromaffin cells and drug effects on release kinetics.
  • Existing methods for cell targeting to electrochemical sensors can be complex and costly.
  • Simultaneous recordings from multiple cells are enabled by CMOS-based sensor arrays.

Purpose of the Study:

  • To develop a reliable, low-cost technique for efficient single-cell targeting to electrode sites for amperometry.
  • To improve the reliability and efficiency of on-chip single-cell amperometry measurements.
  • To validate the technique using live chromaffin cells and assess quantal release properties.

Main Methods:

  • Fabrication of a CMOS-based electrochemical sensor array with SU-8 microwells for cell insulation and trapping.
  • Incorporation of a shifted electrode design for flexible microwell dimensions and shapes.
  • Validation of electrode sensitivity via dopamine injection and parallel recordings of live chromaffin cells.

Main Results:

  • Microwells slightly larger than cells achieved excellent single-cell targeting efficiency.
  • Rapid amperometric spikes without diffusional broadening indicated close cell-electrode contact.
  • Simultaneous recordings revealed significant cell-to-cell variation in spike parameters, ensuring statistical significance.

Conclusions:

  • The developed SU-8 microwell technique offers a reliable and efficient method for on-chip single-cell amperometry.
  • This approach facilitates high-throughput analysis of quantal release events from individual cells.
  • The technique provides valuable insights into cellular release mechanisms and their modulation.