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Updated: Feb 23, 2026

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
Published on: June 30, 2016
Cyclic di-GMP-Responsive Transcriptional Reporter Bioassays in Pseudomonas aeruginosa
Bradley R Borlee1, Grace I Borlee2, Kevin H Martin2
1Department of Microbiology, Immunology and Pathology, Infectious Disease Research Center, Colorado State University, 200 West Lake Street, 2025 Campus Delivery, Fort Collins, CO, 80523, USA. brad.borlee@colostate.edu.
Abstract:
3',5'-cyclic diguanosine monophosphate (cyclic di-GMP) is a bacterial secondary messenger molecule that regulates many important cellular activities and behaviors, such as motility and biofilm formation. While mass spectrometry protocols for quantitative analyses of intracellular cyclic di-GMP concentrations have been developed, they are time intensive, expensive, low-throughput, and incapable of directly monitoring dynamic changes in vivo. In this protocol, we provide a Pseudomonas aeruginosa-specific detailed methodology to assay the intracellular levels of cyclic di-GMP using biological reporters.
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