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Time-resolved Fluorescent Detection for Glucose Using a Complex of Luminescent Layered Titanates and Enzymes
Yuki Sakaguchi1, Tomoka Minamikawa1, Mayuko Yamamuro1
1Department of Biochemistry and Food Science, Faculty of Agriculture, Saga University.
Abstract:
Luminescent europium-doped layered titanates (Eu-TiOx) were synthesized and complexed with horseradish peroxidase (HRP) and glucose oxidase (GOx). The emission of a resultant Eu-TiOx/HRP/GOx complex decreased upon the addition of glucose in the presence of guaiacol. The emission decrease was dependent on the concentrations of glucose, and the detection limit for glucose was 3.1 μM. The proposed system would be promising as a new detection method for glucose.

