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A mouse homeobox containing gene on chromosome 11: sequence and tissue-specific expression
F Meijlink1, R de Laaf, P Verrijzer
1Hubrecht Laboratory, Utrecht, The Netherlands.
Nucleic Acids Research
|September 11, 1987
Summary
Researchers cloned the mouse Hox2.3 gene, a homeobox-containing gene, and analyzed its mRNA sequence. This gene shows high expression in adult mouse tissues and during embryonic development, with varied induction in differentiating EC cells.
Area of Science:
- Developmental Biology
- Genetics
- Molecular Biology
Background:
- Homeobox-containing genes play crucial roles in embryonic development and cell differentiation.
- The Hox gene family is conserved across species and involved in pattern formation.
Purpose of the Study:
- To molecularly clone and characterize the mouse Hox2.3 gene.
- To determine the expression patterns of Hox2.3 in adult mice, embryos, and differentiating cells.
- To investigate the relationship between Hox2.3 and its human homologue.
Main Methods:
- Molecular cloning of cDNA and genomic DNA.
- mRNA sequencing.
- Expression analysis in adult tissues, embryos, and embryonal carcinoma (EC) cells.
Main Results:
- The mouse Hox2.3 gene was successfully cloned and its complete mRNA sequence determined.
- Hox2.3 is highly expressed in adult kidney, testis, and spinal cord, as well as in embryonic spinal cord.
- Differentiation of P19 EC cells induced Hox2.3 expression, with quantitative variations observed across different differentiation pathways.
Conclusions:
- The mouse Hox2.3 gene is a key developmental gene with specific expression patterns.
- Hox2.3 exhibits conserved features with its human homologue, the cl gene.
- The study provides insights into the regulation of Hox2.3 during cellular differentiation.