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Updated: Aug 6, 2026
09:25
Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells
Published on: August 19, 2016
[Microbial inhibitors of phospholipase C]
Prikladnaia Biokhimiia I Mikrobiologiia
|July 1, 1987
Summary
Researchers isolated Clostridium perfringens phospholipase C inhibitors from Streptomyces species. These inhibitors, differing in chemical nature, were purified using ultrafiltration and gel-chromatography.
Area of Science:
- Microbiology
- Biochemistry
- Enzymology
Context:
- Clostridium perfringens phospholipase C (CPE) is a key virulence factor.
- Developing CPE inhibitors is crucial for treating C. perfringens infections.
- Streptomyces species are known producers of bioactive compounds.
Purpose:
- To isolate and characterize inhibitors of Clostridium perfringens phospholipase C.
- To explore the potential of Streptomyces species as a source for these inhibitors.
Summary:
- Inhibitors of Clostridium perfringens phospholipase C were prepared from Streptomyces saracetidus and Streptomyces species filtrates.
- Isolation involved ultrafiltration and polyelectrolyte precipitation.
- The inhibitors from the two strains exhibited different chemical compositions: a polypeptide-glycopeptide complex from S. saraceticus and a high-molecular weight protein from S. species.
Impact:
- This study identifies novel sources and types of C. perfringens phospholipase C inhibitors.
- The findings contribute to the development of new therapeutic strategies against C. perfringens infections.
- Characterization of these inhibitors provides a basis for further biochemical and pharmacological studies.
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