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Updated: Aug 6, 2026

Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells
Published on: August 19, 2016
[Microbial inhibitors of phospholipase C]
Abstract:
Inhibitors of the Clostridium perfringens phospholipase C were prepared from the filtrates of the culture liquids of Streptomyces saracetidus and Streptomyces species using soluble and cross-linked polyelectrolytes. The technological scheme of isolation involves ultrafiltration. The inhibitors produced by the two strains had different chemical nature. The preparation obtained from Str. saraceticus was proved to be a complex of inhibitors that were separated by gel-chromatography into a major polypeptide with a molecular weight of 5500-6500 and a low-molecular weight glycopeptide. The inhibitor obtained from Str. species was found to be a high-molecular weight protein.
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