The basis of differences in thermodynamic efficiency among skeletal muscles

Muscles convert chemical free energy into mechanical work. The energy conversion occurs in 2 steps. First, free energy obtained from oxidation of metabolic substrates (ΔG_S ) is transferred to ATP and, second, free energy from ATP hydrolysis (ΔG_ATP ) is converted into work by myosin cross-bridges. The fraction of ΔG_S transferred to ATP is called mitochondrial efficiency (η_M ) and the fraction of ΔG_ATP converted into work is called cross-bridge efficiency (η_CB ). Overall cross-bridge efficiency varies among muscles from ~20% and 35% and the analysis presented in the current studies shows that this variation is largely due to differences in η_CB whereas η_M is similar (~80%) in all the muscles assessed. There is an inverse, linear relationship between maximum normalised power output and η_CB ; that is, more efficient muscles tend to be less powerful than less efficient muscles. It is proposed that differences in cross-bridge efficiency reflect the extent to which cross-bridges traverse the force-length relationship for attached cross-bridges. In this framework, cross-bridges from tortoise muscle (η_CB  = 45%) produce close to the maximum possible work a cross-bridge can perform in a single attachment cycle.