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Gate-Voltage-Controlled Threading DNA into Transistor Nanopores.

Yuta Kato¹, Naoto Sakashita¹, Kentaro Ishida¹

¹Aoyama-Gakuin University , Sagamihara Campus L617, 5-10-1 Fuchinobe, Chuo, Sagamihara, Kanagawa 252-5258, Japan.

The Journal of Physical Chemistry. B

|September 13, 2017

Summary

This summary is machine-generated.

Researchers developed a novel AC voltage method to control DNA translocation through nanopores using a gate electrode. This technique enables precise manipulation of single DNA molecules for potential applications in biosensing and molecular electronics.

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Area of Science:

Nanotechnology
Molecular Biology
Biophysics

Background:

Controlling the movement of single DNA molecules through nanopores is crucial for various applications.
Conventional methods often rely on external bias voltages, which can be limiting.
Developing simpler, more precise methods for DNA translocation is an ongoing challenge.

Purpose of the Study:

To present a new method for driving DNA translocation using AC voltages applied to a gate electrode within a nanopore.
To demonstrate the ability to thread, oscillate, and translocate single DNA molecules using gate potential modulation.
To offer an alternative to conventional bias voltage methods for biomolecule manipulation.

Main Methods:

Utilized AC voltage waveforms (square and sawtooth) applied to an embedded thin film gate electrode inside a dielectric nanopore.
Investigated DNA molecule behavior (entry, oscillation, threading) in response to different gate voltage modulations.
Measured DNA translocation speed and compared it with theoretical models.

Main Results:

Square waveforms successfully drove single DNA molecules into the nanopore, often resulting in oscillation.
Optimized negative sawtooth voltage pulses effectively threaded a fraction of a DNA molecule into the pore.
The translocation speed of the trapped DNA molecule was comparable to theoretical estimations for low electric fields.

Conclusions:

A simple and effective method for DNA translocation using AC gate voltage modulation in nanopores was demonstrated.
This gate-controlled scheme offers precise manipulation of single DNA molecules, including threading and oscillation.
The findings provide a new tool for controlling biomolecules at the nanoscale, with potential impacts on biosensing and molecular electronics.