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Small RNAs in vancomycin-resistant Enterococcus faecium involved in daptomycin response and resistance
Clara Sinel1, Yoann Augagneur2, Mohamed Sassi2
1University of Caen Normandie, EA4655, Caen, France.
Scientific Reports
|September 13, 2017
Summary
This study identifies novel regulatory RNAs (sRNAs) in vancomycin-resistant Enterococcus faecium, revealing their crucial role in daptomycin antibiotic response and resistance development.
Area of Science:
- Microbiology
- Molecular Biology
- Genomics
Background:
- Vancomycin-resistant Enterococcus faecium (VRE) is a significant cause of hospital-acquired infections.
- Regulatory RNAs (sRNAs) are critical for bacterial adaptation and antibiotic resistance, but are understudied in Gram-positive pathogens like E. faecium.
Purpose of the Study:
- To identify and characterize novel sRNAs in vancomycin-resistant E. faecium.
- To investigate the role of these sRNAs in the response to and development of resistance against daptomycin.
Main Methods:
- Genome-wide identification of sRNAs using genomic and transcriptomic analyses.
- Validation of candidate sRNAs using Northern blot and quantitative PCR (qPCR).
- RNA sequencing (RNA-seq) to analyze gene expression changes with and without daptomycin exposure.
Main Results:
- 61 sRNA candidates were identified, with 10 validated.
- Daptomycin exposure altered the expression of 260 genes, including 7 sRNAs, with sRNA_0160 being experimentally confirmed.
- Stepwise daptomycin resistance mutants showed modified expression of several sRNAs, including sRNA_0160.
Conclusions:
- This is the first genome-wide identification of sRNAs in E. faecium.
- Identified sRNAs are implicated in the antibiotic stress response and daptomycin resistance mechanisms in E. faecium.
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