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Updated: Feb 23, 2026

Autofluorescence Imaging to Evaluate Red Algae Physiology
Published on: February 17, 2023
Subcellular pigment distribution is altered under far-red light acclimation in cyanobacteria that contain chlorophyll
Erica L-W Majumder1, Benjamin M Wolf1, Haijun Liu1
1Departments of Chemistry and Biology, Washington University in St. Louis, One Brookings Dr., St. Louis, MO, 63130, USA.
Abstract:
Far-Red Light (FRL) acclimation is a process that has been observed in cyanobacteria and algae that can grow solely on light above 700 nm. The acclimation to FRL results in rearrangement and synthesis of new pigments and pigment-protein complexes. In this study, cyanobacteria containing chlorophyll f, Synechococcus sp. PCC 7335 and Halomicronema hongdechloris, were imaged as live cells with confocal microscopy. H. hongdechloris was further studied with hyperspectral confocal fluorescence microscopy (HCFM) and freeze-substituted thin-section transmission electron microscopy (TEM). Under FRL, phycocyanin-containing complexes and chlorophyll-containing complexes were determined to be physically separated and the synthesis of red-form phycobilisome and Chl f was increased. The timing of these responses was observed. The heterogeneity and eco-physiological response of the cells was noted. Additionally, a gliding motility for H. hongdechloris is reported.
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