Identification of potential key genes associated with diffuse large B-cell lymphoma based on microarray gene...

Area of Science:

• Oncology
• Genomics
• Bioinformatics

Background:

• Diffuse large B-cell lymphoma (DLBCL) is an aggressive non-Hodgkin lymphoma.
• Identifying key molecular players in DLBCL pathogenesis is crucial for developing effective therapies.

Purpose of the Study:

• To screen key genes, microRNAs (miRNAs), and transcription factors (TFs) associated with DLBCL.
• To explore potential therapeutic targets for DLBCL progression.

Main Methods:

• Differential gene expression analysis using Limma on GSE56315 dataset.
• Functional and pathway enrichment analyses of differentially expressed genes (DEGs).
• Construction of functional interaction (FI) networks, TF-target-miRNA integrated networks.

Main Results:

• Identified 4,495 DEGs between DLBCL and normal tonsil samples.
• Enriched pathways include chemokine signaling, phosphatidylinositol signaling, and RNA degradation.
• Highlighted key miRNAs (miR-21-5p, miR-155, miR-17-5p), TF (STAT1), and DEGs (NUF2, CCR1, PIK3R1, SMC1A, FOXK1, CNOT6L) in integrated networks.

Conclusions:

• NUF2, CCR1, PIK3R1, SMC1A, FOXK1, and CNOT6L are potentially closely associated with DLBCL pathogenesis.
• The identified molecular players represent potential therapeutic targets for DLBCL.