Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.2K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.2K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

5.7K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.7K
iPS Cell Differentiation01:22

iPS Cell Differentiation

3.2K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
3.2K
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

3.5K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Final overall survival analysis and exploratory biomarker study from JUPITER-06: a randomized phase III trial of toripalimab plus chemotherapy in advanced esophageal squamous-cell carcinoma.

Annals of oncology : official journal of the European Society for Medical OncologyĀ·2026
Same author

Corrigendum to "AdipoRon ameliorates anxiety- and depression-like behaviors in chronic restraint-stressed mice via AMPK-PPARĪ±-BDNF-TrkB signaling" [Eur. J. Pharmacol. (2026) 1011 178468 doi: 10.1016/j.ejphar.2025.178468].

European journal of pharmacologyĀ·2025
Same author

The Remineralization Potential of Resveratrol and Cucurbit[<i>n</i>]uril.

Journal of dental researchĀ·2025
Same author

[Long-term prognostic follow-up analysis of multiphasic myelin oligodendrocyte glycoprotein antibody-associated disease in children].

Zhonghua er ke za zhi = Chinese journal of pediatricsĀ·2025
Same author

Stable Nitrogen Isotope Analysis of Amino Acids by Orbitrap Mass Spectrometry: Application for Extraterrestrial Samples.

Rapid communications in mass spectrometry : RCMĀ·2025
Same author

<i>Cryphodera guangdongensis</i> n. sp., (Nematoda: Heteroderidae), a new species of cystoid nematode from roots and surrounding soil of <i>Schima superba</i> in Guangdong, China.

Journal of helminthologyĀ·2025

Related Experiment Video

Updated: Feb 23, 2026

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
10:52

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Published on: March 22, 2024

2.3K

Functional Odontoblastic-Like Cells Derived from Human iPSCs.

H Xie1, N Dubey1, W Shim2,3

  • 11 Oral Sciences, Faculty of Dentistry, National University of Singapore, Singapore.

Journal of Dental Research
|September 13, 2017
PubMed
Summary

Human induced pluripotent stem cells (iPSCs) derived from dental pulp stem cells (DPSCs) can successfully differentiate into functional odontoblasts. These iPSCs offer a promising cell source for dental pulp research and material bioactivity testing.

Keywords:
biomaterial(s)cell differentiationdentinogenesisodontoblast(s)pulp biologytissue engineering

More Related Videos

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

27.3K
Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
03:45

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

3.4K

Related Experiment Videos

Last Updated: Feb 23, 2026

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
10:52

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Published on: March 22, 2024

2.3K
Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

27.3K
Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
03:45

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

3.4K

Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Dental Research

Background:

  • Induced pluripotent stem cells (iPSCs) possess self-renewal and differentiation capabilities.
  • Dental pulp stem cells (DPSCs) are a potential source for generating iPSCs.
  • Odontoblasts are crucial for dentin formation and pulp health.

Purpose of the Study:

  • To investigate the potential of DPSCs-derived iPSCs to differentiate into functional odontoblasts.
  • To assess the odontogenic differentiation capacity of iPSCs after long-term expansion.
  • To evaluate iPSCs as a cell source for dental pulp research and material testing.

Main Methods:

  • DPSCs were reprogrammed into iPSCs using specific reprogramming factors (OCT-4, SOX2, KLF4, LIN28, L-MYC).
  • iPSCs were characterized in vitro (gene/protein expression) and in vivo (teratoma formation).
  • iPSCs seeded on scaffolds were implanted subcutaneously in mice to assess in vivo dentin formation.
  • In vitro odontogenic differentiation was induced using specific media and bioactive cement extract, comparing early and late passage iPSCs with DPSCs.

Main Results:

  • Reprogrammed iPSCs exhibited characteristic pluripotency markers and formed tissues from three germ layers in vivo.
  • Subcutaneous implantation of iPSCs on scaffolds resulted in the formation of pulp-like tissue with tubular dentin.
  • Long-term expanded iPSCs maintained odontoblastic differentiation markers and mineralization potential, unlike late-passage DPSCs.

Conclusions:

  • Human iPSCs derived from DPSCs can be successfully differentiated into functional odontoblasts.
  • iPSCs represent a stable and expandable cell source for generating odontoblasts.
  • This study highlights the utility of iPSCs for dental pulp regeneration research and evaluating biomaterials.