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Updated: Feb 23, 2026

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
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Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

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Application of MALDI-TOF MS Systems in the Rapid Identification of Campylobacter spp. of Public Health Importance

Ying-Hsin Hsieh1, Yun F Wang2, Hercules Moura3

  • 1U.S. Food and Drug Administration, Microbiological Sciences Branch, Southeast Food and Feed Laboratory, 60 8th St NE, Atlanta, GA 30309.

Journal of AOAC International
|September 14, 2017
PubMed

Insights

Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) MS offers rapid identification of Campylobacter species, overcoming limitations of traditional DNA-based methods. This technique can improve diagnostics for this important gastrointestinal pathogen.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Diagnostic Technology

Background:

  • Campylobacteriosis, a common gastrointestinal illness, is often underdiagnosed due to diagnostic limitations.
  • Current DNA-based methods like 16S ribosomal RNA (rRNA) sequencing for Campylobacter identification are time-consuming and require high-quality DNA.
  • Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) Mass Spectrometry (MS) is a promising rapid identification method using intact bacterial cells.

Purpose of the Study:

  • To evaluate the efficacy of two commercial MALDI-TOF MS platforms for rapid identification of Campylobacter species.
  • To compare MALDI-TOF MS identification with 16S rRNA sequencing for accuracy.

Main Methods:

  • Analysis of 24 reference strains of 16 Campylobacter spp. and five unknown clinical isolates.
  • Utilized two MALDI-TOF MS systems: bioMérieux VITEK® MS and Bruker Biotyper.
  • Confirmed species identification of clinical isolates using 16S rRNA sequencing.

Main Results:

  • Both MALDI-TOF MS platforms successfully identified key Campylobacter species, including C. jejuni, C. coli, C. lari, and C. fetus.
  • MALDI-TOF MS provided rapid identification without the need for DNA extraction or purification.

Conclusions:

  • MALDI-TOF MS is a viable and efficient technique for the rapid identification of Campylobacter species.
  • This technology holds potential for improving the diagnosis of Campylobacter infections of public health significance.

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