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Published on: October 31, 2014
Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants
Alexander Grosse-Honebrink1, Katrin M Schwarz1, Hengzheng Wang1
1Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
Abstract:
Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transformation of the solvent producing organism Clostridium pasteurianum by three to four orders of magnitude.

