Related Experiment Video
Updated: Feb 23, 2026
08:35
Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
Published on: September 11, 2014
18.0K
[Nascent Polypeptide-Associated Complex as Tissue-Specific Cofactor during Germinal Cell Differentiation in
G L Kogan1, N V Akulenko1, Yu A Abramov1
1Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia.
Molekuliarnaia Biologiia
|September 14, 2017
Summary
Spermatogenesis involves changes in gene expression. In Drosophila, the nascent polypeptide-associated complex (NAC) is newly found to be tissue-specific in spermatocytes, potentially regulating protein expression for later stages.
Area of Science:
- Cell Biology
- Developmental Biology
- Molecular Biology
Background:
- Spermatogenesis involves transcriptional reprogramming as spermatogonia differentiate into spermatocytes.
- The nascent polypeptide-associated complex (NAC) is a known chaperone involved in co-translational protein folding.
Purpose of the Study:
- To investigate the role of NAC during spermatogenesis.
- To identify tissue-specific co-translational cofactors in multicellular eukaryotes.
Main Methods:
- Analysis of gene expression during Drosophila spermatogenesis.
- Detection of NAC association with ribosomes in specific tissues.
Main Results:
- A significant increase in the expression of the beta-subunit of NAC associated with ribosomes was observed during spermatocyte development.
- This marks the first detection of a tissue-specific co-translational NAC cofactor in multicellular eukaryotes.
Conclusions:
- Spermatocyte-specific NAC may play a crucial role in modulating protein expression.
- This regulation is likely essential for the successful progression of subsequent spermatogenesis stages.
More Related Videos
Related Concept Videos
02:04Polytene Chromosomes
11.1K
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
11.1K
02:45The Ratio of X Chromosome to Autosomes
9.9K
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
9.9K