Aestuarium zhoushanense gen. nov., sp. nov., Isolated from the Tidal Flat

A gram-stain-negative, aerobic, ovoid or short rod-shaped, and non-motile strain, designed G7^T was isolated from a tidal flat sample collected from the coast of East Sea in Zhoushan, China. Strain G7^T grew at 4-40 °C and pH 6.0-9.0 (optimum, 28 °C and pH 7.5) and with 0-7% (w/v) NaCl (optimum, 1%). The predominant respiratory quinone was Q-10 and the major fatty acids (>10%) identified were C_18:1 ω7c, C_16:0 and summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c). The polar lipids of strain G7^T consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, and four unidentified lipids. The genomic DNA G+C content was 56.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain G7^T formed a distinct lineage belonging to the Roseobacter clade of the family Rhodobacteraceae. On the basis of morphological, physiological, and chemotaxonomic characteristics, together with the results of phylogenetic analysis, strain G7^T is described as a novel species in a new genus, for which the name Aestuarium zhoushanense gen. nov., sp. nov. (type strain G7^T = MCCC 1K03229^T = KCTC 52584^T) is proposed.