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Updated: Feb 23, 2026
Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
LC-MS/MS analysis of lipidized analogs of prolactin-releasing peptide utilizing a monolithic column and simple sample
Jana Zemenová1,2, David Sýkora1, Anna Freislebenová1
1Department of Analytical Chemistry, University of Chemistry & Technology Prague, Technická 5, 16628 Prague, Czech Republic.
Aim:
Novel compounds for obesity treatment are currently being studied employing lipidized analogs of anorexigenic neuropeptides. Various analogs of prolactin-releasing peptide have demonstrated their ability to decrease food intake. Adequate analytical tools are required to support corresponding research. Methodology & results: An analytical method was developed that includes simple dilution of plasma samples prior to liquid chromatography-mass spectrometry and employs a monolithic column for the determination of lipidized analogs of prolactin-releasing peptide in complex biological samples. A multiple reaction monitoring approach was applied that included matrix calibration and an internal standard and produced a linear calibration range 20-200 ng ml-1 in rat and macaque plasma samples.
Conclusion:
A straightforward, simple and reliable analytical method was developed satisfying major validation criteria.
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