Association between endothelin‑1 and fibromyalgia syndrome

Area of Science:

• Genetics and Molecular Biology
• Rheumatology
• Cardiovascular Physiology

Background:

• Fibromyalgia syndrome (FMS) presents as widespread pain and tenderness.
• Patients with FMS may experience cold extremities and vasospasm.
• Endothelin-1 (EDN1), a potent vasoconstrictor, is produced by endothelial cells.

Purpose of the Study:

• To investigate the association between plasma Endothelin-1 (EDN1) levels and single-nucleotide polymorphism (SNP; rs1800541) of the EDN1 gene with Fibromyalgia syndrome (FMS).
• To explore the relationship between EDN1 expression and SNP with FMS clinical variables.

Main Methods:

• Plasma EDN1 levels were quantified using ELISA.
• EDN1 gene SNP genotypes were determined via polymerase chain reaction-high-resolution melting curve analysis.
• Comparison of EDN1 levels and genotypes between FMS patients and healthy controls.

Main Results:

• Significantly elevated plasma EDN1 levels were observed in FMS patients compared to controls.
• The TG genotype and G allele of the EDN1 gene were associated with an increased risk of FMS.
• FMS patients with the TG genotype and/or T allele showed higher plasma EDN1 levels.

Conclusions:

• Plasma EDN1 levels are significantly higher in patients with Fibromyalgia syndrome.
• The EDN1 gene SNP (rs1800541) is associated with susceptibility to FMS.
• EDN1 may contribute to the pathophysiology of FMS, potentially influencing vasoconstriction and pain.