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The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability
Published on: April 28, 2022
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Iron Absorption from Three Commercially Available Supplements in Gastrointestinal Cell Lines
Francesca Uberti1, Vera Morsanuto2, Sabrina Ghirlanda3
1Laboratory of Physiology, Department of Translational Medicine, University of Eastern Piedmont, via Solaroli 17, 28100 Novara, Italy. francesca.uberti@med.uniupo.it.
Nutrients
|September 14, 2017
Summary
Fisioeme® demonstrates superior cell viability compared to Sideral® and iron bisglycinate. This iron supplement is most suitable for both gastric and intestinal epithelial cells, indicating enhanced biocompatibility.
Area of Science:
- Biochemistry
- Cell Biology
- Nutritional Science
Background:
- Iron deficiency anemia is a global health concern requiring effective oral iron supplements.
- Chelated iron, such as iron bisglycinate, is commonly used for improved absorption and reduced gastrointestinal side effects.
- Novel iron formulations are continuously being developed to enhance bioavailability and cellular compatibility.
Purpose of the Study:
- To compare the absorption characteristics and biocompatibility of two novel iron-based dietary supplements (Fisioeme® and Sideral®) against iron bisglycinate.
- To evaluate the impact of these iron formulations on cellular viability and absorption mechanisms in gastric and intestinal cell models.
Main Methods:
- Utilized Caco-2 and GTL-16 cell lines as models for human intestinal and gastric epithelial cells, respectively.
- Assessed iron absorption kinetics by analyzing the expression of divalent metal transporter 1 (DMT1).
- Investigated cellular responses to iron formulations over various time points, including short-term and long-term effects, and assessed extrusion mechanisms.
Main Results:
- Fisioeme® significantly enhanced cell viability compared to control, Sideral®, and iron bisglycinate.
- The study identified differences in absorption kinetics and cellular responses among the tested iron formulations.
- Fisioeme® demonstrated a sustained beneficial effect on cell viability, suggesting favorable interaction with epithelial cells.
Conclusions:
- Fisioeme® exhibits superior biocompatibility and a positive impact on cell viability for both gastric and intestinal epithelial cells.
- The findings suggest Fisioeme® is a promising iron formulation for dietary supplements due to its enhanced suitability for gastrointestinal cells.
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