Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

HLA class I- like antigen expression on human leukemic cells.

D Peruccio1, T Crepaldi, E Lovisone

  • 1Dipartimento di Genetica, Biologia e Chimica Medica, University of Torino, Italy.

Tissue Antigens
|August 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of Fragmentation Functions for Jets Dominated by Light Quarks and Gluons from pp and Pb+Pb Collisions in ATLAS.

Physical review letters·2019
Same author

Combination of Searches for Invisible Higgs Boson Decays with the ATLAS Experiment.

Physical review letters·2019
Same author

Measurement of differential cross-sections of a single top quark produced in association with a <i>W</i> boson at <math> </math> with ATLAS.

The European physical journal. C, Particles and fields·2019
Same author

Search for the direct production of charginos and neutralinos in final states with tau leptons in <math> </math> <i>pp</i> collisions with the ATLAS detector.

The European physical journal. C, Particles and fields·2019
Same author

Measurement of quarkonium production in proton-lead and proton-proton collisions at <math></math> with the ATLAS detector.

The European physical journal. C, Particles and fields·2019
Same author

Measurement of longitudinal flow decorrelations in Pb+Pb collisions at <math> </math> and 5.02 TeV with the ATLAS detector.

The European physical journal. C, Particles and fields·2019

Researchers analyzed HLA class I-like molecules on leukemia cells. These molecules, absent in normal lymphocytes, were found on 50% of acute leukemias, suggesting they may indicate leukemic blast activation.

Area of Science:

  • Immunogenetics
  • Hematology
  • Molecular Biology

Background:

  • Human Leukocyte Antigen (HLA) class I molecules are crucial for immune recognition.
  • Aberrant expression of HLA molecules can occur in various hematological malignancies.
  • Beta-2-microglobulin (β2M) is a component of functional HLA class I molecules.

Purpose of the Study:

  • To investigate the expression of HLA class I-like molecules on human acute and chronic leukemic cells.
  • To determine if these molecules can serve as markers for leukemic blast activation or maturation.

Main Methods:

  • Analysis of HLA class I-like molecule expression using complement-dependent lymphocytotoxicity assays.
  • Utilized platelet-absorbed alloantisera recognizing HLA-linked, β2M-associated molecules.

Related Experiment Videos

  • Examined expression on various acute leukemias (cALL, T-ALL, AML) and chronic lymphocytic leukemias (B-CLL).

    • Main Results:

    • HLA class I-like molecules were detected on 50% of acute leukemias (cALL, T-ALL, AML), irrespective of differentiation lineage.
    • Chronic lymphocytic leukemias (B-CLL) consistently showed negative reactivity for these molecules.
    • The detected molecules were absent on the patient's normal lymphocytes.

    Conclusions:

    • The expression of β2M-associated HLA molecules on leukemic cells may serve as a marker.
    • These molecules could indicate leukemic blast activation and/or the maturation state.
    • Differential expression patterns observed between acute and chronic leukemias warrant further investigation.