Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Assembly of Signaling Complexes

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...

Amplifying Signals via Enzymatic Cascade

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...

Transducer Mechanism: Enzyme-Linked Receptors

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:

Interactions Between Signaling Pathways

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Transcriptomic analysis of tissue-resident memory T cells of the fallopian tube reveals a precursor immune surveillance network for ovarian cancer prevention.

Nature communications·2026

Same author

Specific modulation of CRISPR transcriptional activators through RNA-sensing guide RNAs in mammalian cells and zebrafish embryos.

eLife·2025

Same author

Oxford Classic-Defined EMT Risk Stratification of High-Grade Serous Ovarian Cancer for Guiding Treatment Decisions.

Clinical cancer research : an official journal of the American Association for Cancer Research·2025

Same author

LCN2-mediated ferroptosis resistance in tissue homeostasis and early-stage tumorigenesis of the fallopian tube epithelium.

iScience·2025

Same author

mTOR-mediated p62/SQSTM1 stabilization confers a robust survival mechanism for ovarian cancer.

Cancer letters·2025

Same author

3D Microtumors Representing Ovarian Cancer Minimal Residual Disease Respond to the Fatty Acid Oxidation Inhibitor Perhexiline.

Advanced healthcare materials·2025

Search research articles

Related Experiment Video

Updated: Feb 23, 2026

Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy

Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy

Published on: September 29, 2016

Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors.

Toni A Baeumler¹, Ahmed Ashour Ahmed², Tudor A Fulga¹

¹Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, UK.

|September 14, 2017

Summary

Researchers developed a new synthetic receptor platform using CRISPR/Cas9 (dCas9) technology. This dCas9-synR system allows designer cells to sense signals and control gene expression for advanced cellular engineering.

Keywords:

CRISPR GPCR RTK chimeric receptors dCas9-VP64 dCas9-synR genome engineering split Cas9 synthetic receptors transcriptional programs

More Related Videos

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

Identification of Functional Protein Regions Through Chimeric Protein Construction

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

Related Experiment Videos

Last Updated: Feb 23, 2026

Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy

Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy

Published on: September 29, 2016

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

Identification of Functional Protein Regions Through Chimeric Protein Construction

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

Area of Science:

Synthetic biology
Molecular and cellular biology
Biotechnology

Background:

Synthetic receptors are crucial for engineering cells to perform specific functions.
Current limitations exist in the programmability and adaptability of synthetic receptors.

Purpose of the Study:

To develop a novel, modular synthetic receptor platform based on nuclease-deficient CRISPR/Cas9 (dCas9).
To demonstrate the adaptability and functionality of this platform for cellular engineering applications.

Main Methods:

Engineered chimeric receptors integrating a dCas9 signal transduction module with native ectodomain scaffolds.
Tested receptor performance for OFF/ON state transitions and dose-dependent activation.
Programmed receptors to link disease markers with multi-gene expression circuits.

Main Results:

The dCas9 synthetic receptor (dCas9-synR) architecture was successfully adapted to various scaffolds.
Receptors exhibited stringent OFF/ON state transitions and dose-dependent activation.
Demonstrated programming of disease markers to control therapeutically relevant gene circuits.

Conclusions:

The modular dCas9-synR platform offers a generalizable blueprint for next-generation synthetic receptors.
This technology enables the creation of designer cells with complex, customizable functions.
Advances cellular engineering by providing a versatile tool for signal transduction and gene regulation.