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Updated: Feb 23, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Targeted Metagenome Based Analyses Show Gut Microbial Diversity of Inflammatory Bowel Disease patients
Zhibing Qiu1, Haijing Yang1, Lan Rong1
1Department of Digestive Diseases, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Rd., Shanghai, 200040 China.
Abstract:
Inflammatory bowel disease (IBD) is a multifactorial disease including both genetic and environmental factors. We compared the diversity of intestinal microbesamong a cohort of IBD patients to study the microbial ecological effects on IBD. Fecal samples from patients were sequenced with next generation sequence technology at 16S rDNA region. With statistical tools, microbial community was investigated at different level. The gut microbial diversity of Crohn's disease (CD) patients and colonic polyp (CP) patients significantly different from each other. However, the character of ulcerative colitis (UC) patients has of both CD and CP features. The microbial community from IBD patients can be very different (CD patient) or somewhat similar (UC patients) to non-IBD patients. Microbial diversity can be an important etiological factor for IBD clinical phenotype.
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