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Updated: Feb 23, 2026

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
Published on: September 28, 2022
Cyclic peptide production using a macrocyclase with enhanced substrate promiscuity and relaxed recognition
Cristina N Alexandru-Crivac1, Christian Umeobika2, Niina Leikoski3
1Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen AB24 3UE, UK. l.trembleau@abdn.ac.uk w.houssen@abdn.ac.uk and Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, UK.
Abstract:
Macrocyclic peptides have promising therapeutic potential but the scaling up of their chemical synthesis is challenging. The cyanobactin macrocyclase PatGmac is an efficient tool for production but is limited to substrates containing 6-11 amino acids and at least one thiazoline or proline. Here we report a new cyanobactin macrocyclase that can cyclize longer peptide substrates and those not containing proline/thiazoline and thus allows exploring a wider chemical diversity.

