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Plasmonic cell nanocoating: a new concept for rapid microbial screening.

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Summary

Rapidly coat microbial cells with gold nanoparticles using specific surface molecules like disulfide-bond-containing proteins and chitin. This fast nanocoating method enables quick detection and quantification of bacteria and fungi.

Keywords:
Cell nanocoatingChitinDisulfide-bond containing (Dsbc) surface proteinsGold nanoparticlesMicrobial screening

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Area of Science:

  • Microbiology
  • Nanotechnology
  • Biotechnology

Background:

  • Nanocoating microbial cells with gold nanostructures offers novel properties for control, study, and detection.
  • Current methods like layer-by-layer (LbL) deposition are time-consuming and rely on nonspecific interactions, limiting diagnostic applications.

Purpose of the Study:

  • To develop a rapid and specific method for microbial cell nanocoating using gold nanoparticles.
  • To enable fast screening and quantification of bacteria and fungi.

Main Methods:

  • Utilized specific surface molecules on microbial cells, including disulfide-bond-containing (Dsbc) proteins and chitin, for gold nanoparticle interaction.
  • Employed a rapid activation and interaction process, significantly faster than LbL deposition.
  • Used plasmonics and fluorescence as transduction methods for detection.

Main Results:

  • Achieved cell nanocoating within seconds by targeting specific surface molecules.
  • Enabled specific microbial screening and quantification of bacteria (E. coli) in 5 minutes and fungi (M. circinelloides) in 30 minutes.
  • Demonstrated a low limit of detection (<35 cfu mL⁻¹ for E. coli, <1500 cfu mL⁻¹ for M. circinelloides) using a hand-held reader.

Conclusions:

  • Developed a novel, rapid, and specific nanocoating strategy for microbial cells.
  • This approach significantly reduces detection and quantification times for bacteria and fungi.
  • The method holds potential for advanced microbial diagnostics and applications.