Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

759
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
759
Next-generation Sequencing03:00

Next-generation Sequencing

99.3K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
99.3K
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

18.7K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
18.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The impact of multiplex panel testing on ascertainment of pertussis-attributable, non-infant deaths: national surveillance from England.

Journal of clinical microbiology·2026
Same author

Antibiotic non-susceptibility associated serotypes of invasive pneumococcal disease - a nationwide population study from Switzerland, 2012-2022.

Pneumonia (Nathan Qld.)·2026
Same author

Heavy Metal Toxicity in Clinical and Environmental Health: Sources, Mechanisms, Diagnostics, and Evidence-Based Management of Mercury, Lead, Cadmium, and Arsenic.

International journal of molecular sciences·2026
Same author

An infant nasal microbial gene atlas uncovers intervention-driven microbiome shifts and salt-resistant pathogen expansion.

Cell host & microbe·2026
Same author

The impact of nutritional, environmental, and lifestyle factors on neurological disorders: therapeutic implications and mechanistic insights.

Frontiers in pharmacology·2026
Same author

Effect of fluorinated substituents of Zn phthalocyanine: nanoencapsulation and phototoxicity.

International journal of pharmaceutics·2026

Related Experiment Video

Updated: Feb 22, 2026

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
06:06

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

Published on: September 11, 2020

2.2K

Pneumococcal 23B Molecular Subtype Identified Using Whole Genome Sequencing.

Georgia Kapatai1, Carmen L Sheppard1, Lukas J Troxler2

  • 1Respiratory and Vaccine Preventable Bacteria Reference Unit, Public Health England, National Infection Service, London, United Kingdom.

Genome Biology and Evolution
|September 16, 2017
PubMed
Summary
This summary is machine-generated.

A novel genetic variant of Streptococcus pneumoniae serotype 23B, named 23B1, was identified through whole genome sequencing. Despite genetic differences, 23B1 shows no serological or structural variation, yet it has significantly restructured the UK 23B population.

Keywords:
Streptococcus pneumoniaecapsular polysaccharidegenotypeserotypewhole genome sequencing

More Related Videos

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction
04:25

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction

Published on: February 24, 2014

46.7K
Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
22:10

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

Published on: June 28, 2013

13.7K

Related Experiment Videos

Last Updated: Feb 22, 2026

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
06:06

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

Published on: September 11, 2020

2.2K
Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction
04:25

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction

Published on: February 24, 2014

46.7K
Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
22:10

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

Published on: June 28, 2013

13.7K

Area of Science:

  • Microbiology
  • Genomics
  • Vaccinology

Background:

  • The polysaccharide capsule of Streptococcus pneumoniae is a key virulence factor and vaccine target.
  • There are 92 known pneumococcal serotypes, with variation arising from the capsular polysaccharide synthesis (CPS) locus.
  • Genetic variation in the CPS locus does not always lead to novel serotypes.

Purpose of the Study:

  • To describe a novel genotypic variant of the serotype 23B CPS operon.
  • To characterize the genetic and phenotypic properties of this variant.
  • To investigate its evolutionary lineage and population impact.

Main Methods:

  • Whole genome sequencing of Streptococcus pneumoniae isolates.
  • Sequence homology analysis of the CPS operon.
  • Serological and structural analysis of capsular polysaccharides.
  • Phylogenetic and eBURST analysis for evolutionary insights.

Main Results:

  • A novel genotypic subtype, 23B1, with ~70% homology to 23B CPS sequence was identified.
  • High sequence variability was observed in eight cps genes.
  • No serological or structural distinction was found between 23B and 23B1.
  • Phylogenetic analysis revealed a distinct lineage for 23B1 with multiple global clones.
  • A significant increase in 23B1 (ST2372) prevalence was observed in UK isolates starting in 2011, reaching 50% by 2013.

Conclusions:

  • The 23B1 variant represents a genotypic subtype without phenotypic impact, thus not a novel serotype.
  • Despite lacking phenotypic novelty, 23B1 has driven significant genetic restructuring within the UK serotype 23B population.
  • This highlights the importance of genomic surveillance for understanding pathogen evolution and population dynamics.