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Related Concept Videos

High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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Chromatographic Methods: Classification01:12

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Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
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High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

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In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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Related Experiment Video

Updated: Feb 22, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
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An analysis method for flavan-3-ols using high performance liquid chromatography coupled with a fluorescence

Liuqing Wang1, Yoko Yamashita1, Akiko Saito2

  • 1Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.

Journal of Food and Drug Analysis
|September 16, 2017
PubMed
Summary

This study developed a highly sensitive HPLC-fluorescence method to analyze procyanidins, revealing their bioavailability and presence in plant foods. The new method is 1000 times more sensitive than traditional UV detection for flavan-3-ols.

Keywords:
bioavailabilityepicatechinfluorescence detectionhigh performance liquid chromatographyprocyanidin

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Last Updated: Feb 22, 2026

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Area of Science:

  • Analytical Chemistry
  • Food Science
  • Pharmacology

Background:

  • Procyanidins, a class of flavan-3-ols found in plant foods, possess beneficial physiological activities.
  • Understanding procyanidin bioavailability is limited by the lack of sensitive analytical methods.
  • Existing methods often lack the sensitivity required for accurate quantification in biological and food matrices.

Purpose of the Study:

  • To develop and validate a highly sensitive and convenient analytical method for determining procyanidin content.
  • To apply the developed method for analyzing procyanidins in food materials and biological samples.
  • To investigate the bioavailability of procyanidins in vivo.

Main Methods:

  • High-performance liquid chromatography (HPLC) coupled with a fluorescence detector was employed.
  • The method achieved significantly low limits of detection (LOD) and quantification (LOQ) for various flavan-3-ols.
  • Procyanidin content was measured in black soybean seed coat extracts (BE) and in mouse plasma after oral administration.

Main Results:

  • The developed HPLC-fluorescence method demonstrated approximately 1000-fold higher sensitivity compared to conventional HPLC-UV detection.
  • Procyanidin content in BE varied based on agricultural conditions, with intertillage yielding the highest amounts.
  • In mice, procyanidins up to the tetramer level were detected in plasma, primarily in conjugated forms.

Conclusions:

  • A novel, highly sensitive analytical method for flavan-3-ols was successfully established.
  • The method enables accurate quantification of procyanidins in diverse samples, including food and biological matrices.
  • This advancement facilitates further research into the bioavailability and health effects of procyanidins.