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Related Experiment Video

Updated: Feb 22, 2026

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Published on: July 8, 2021

Matrisome Profiling During Intervertebral Disc Development And Ageing.

Joana Caldeira^1,2,3, Cátia Santa^4,5, Hugo Osório^6,7,8

¹i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal. joana.caldeira@ineb.up.pt.

Scientific Reports

|September 16, 2017

Summary

This study profiled the extracellular matrix in bovine nucleus pulposus (NP) during development and aging. It identified key proteins that change with age, offering insights into intervertebral disc (IVD) degeneration and potential regeneration strategies.

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Last Updated: Feb 22, 2026

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Published on: July 8, 2021

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

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Published on: February 14, 2021

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

Published on: April 11, 2017

Area of Science:

Biochemistry
Cell Biology
Biomaterials Science

Background:

Intervertebral disc (IVD) degeneration is a primary cause of low back pain, characterized by extracellular matrix (ECM) depletion and nucleus pulposus (NP) extrusion.
Age-associated changes in the IVD are significant, yet a comprehensive understanding of ECM composition throughout development and aging is lacking.

Purpose of the Study:

To define the nucleus pulposus (NP) matrisome across different developmental and aging stages in bovine discs.
To establish a foundational database of healthy disc ECM composition to understand homeostasis and age-related changes.

Main Methods:

iTRAQ Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) analysis was employed on fetal, young, and old bovine NP samples.
Proteomic profiling was used to identify and quantify ECM proteins, followed by independent validation of key findings.

Main Results:

Distinct ECM profiles were observed across fetal, young, and old bovine NPs.
Specific enrichments included Collagen XII and XIV in fetal NPs, Fibronectin and Prolargin in older NPs, and Collagen XI in young NPs.

Conclusions:

This study presents the first comprehensive matrisome database for healthy IVDs during development and aging.
The identified proteins and pathways are crucial for understanding disc homeostasis and may serve as targets for treating age-associated IVD degeneration or for regenerative therapies.